Speaker: Ali Jaafar Sadeq (HUN-REN Wigner RCP SZFI)
Title: Confocal Raman micro-spectroscopy of collagen-containing tissues: the impact of optical clearing
Date: Tuesday, 23 January 2024, 10:00,
Place: KFKI Campus, Bldg. 1, 2nd floor, Conference Room
Abstract:
Raman spectroscopy as one of the optical spectroscopic methods provides valuable information about the molecular structure and conformation, which gains great attention for diagnosis and monitoring of biological objects. All biomedical optics including Raman spectroscopy are suffering from strong light scattering or limited in-depth light penetration. These issues pose significant hindrance to the practical application of the optical techniques in clinical practice. In the 90s of the last century, the optical clearing technique based on the immersion of the tissue into optical clearing agent was introduced and developed to enhance the detection depth using optical techniques. In this thesis work, I studied the impact of optical clearing method on collagen-related Raman peaks to control the optical properties of biological objects, specifically ex vivo porcine dura mater and skin-dermis in-depth using confocal Raman micro-spectroscopy. I determined the impact of pure glycerol as an optical clearing agent on the intensity of collagen-related Raman peak, as well as on dehydration and dissociation of the collagen using confocal Raman micro-spectroscopy at various depths ranging from 0 to 250 μm and treatment times [1]. In addition, the diffusivity of 50% glycerol and the changes in the water content within the collagen of the dura mater were monitored at various depths and treatment times [2]. The effect of the optical clearing on the Raman spectra recorded with 633- and 785-nm excitations in ex vivo porcine skin-dermis was also studied at different depths and treatment times, together with the influence of dimethyl sulfoxide as an enhancer additive [3].
[1] Ali Jaafar, Maxim E. Darvin, Valery V. Tuchin and Miklós Veres; Confocal Raman Micro-Spectroscopy for Discrimination of Glycerol Diffusivity in Ex vivo porcine dura mater; Life 2022, 12(10), 1534; https://doi.org/10.3390/life12101534; Q2; IF: 3.2.
[2] Ali Jaafar, Roman Holomb, Anton Y. Sdobnov, Zsombor Ocskay, Zoltan Jakus, Valery V. Tuchin and Miklos Veres; Ex vivo confocal Raman microspectroscopy of porcine dura mater supported by optical clearing; J.Biophotonics.2022; 15:e202100332.https://doi.org/10.1002/jbio.202100332; Q1; IF:2.8.
[3] Ali Jaafar, Malik H. Mahmood, Roman Holom, Laszló Himics, Tamas Vaczi, Anton Y. Sdobnov|, Valery V. Tuchin and Miklós Veres; Ex-vivo confocal Raman microspectroscopy of porcine skin with 633/785-NM laser excitation and optical clearing with glycerol/water/DMSO solution; Journal of Innovative Optical Health Sciences Vol. 14, No. 05, 2142003,2021, https://doi.org/10.1142/S1793545821420037; Q2; IF: 2.5.
